Mouse Anti-MSLN Recombinant Antibody (HPAB-0886-FY)
CAT#: HPAB-0886-FY
This product is a recombinant mouse antibody that recognizes MSLN. The antibody was purified by affinity chromatography. It can be used for FC and other applications.
Tested Data
Gene Expression
DB
Figure 1 Mouse Anti-Human MSLN Antibody (HPAB-0886-FY) in DB
Dot Blot analysis of HPAB-0886-FY was performed by coating with Human MSLN Protein (His Tag).
HPAB-0886-FY incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 2000)
ELISA
Figure 2 Mouse Anti-Human MSLN Antibody (HPAB-0886-FY) in ELISA
ELISA analysis of HPAB-0886-FY was performed by coating with Human MSLN Protein (His Tag). Then blocked with BSA and incubated with HPAB-0886-FY. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 3 Mouse Anti-Human MSLN Antibody (HPAB-0886-FY) in WB
Western blot analysis of HPAB-0886-FY was performed by loading Human MSLN Protein (His Tag).
HPAB-0886-FY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-mouse IgG as a secondary antibody (1: 2000)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
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Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17172/1276_A4_4_selected.jpg
Normal Tissue
Figure 2 Fallopian tube
Ciliated cells (cilia axoneme)
Staining:
High
Intensity: Strong
Ciliated cells (tip of cilia)
Staining:
High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2216/6076_A_7_7.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, EFO-21, HeLa, hTCEpi, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MSLN
- Species Reactivity
- Human, Cynomolgus monkey
- Applications
- FC
- Related Disease
- Lung Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0886-FY, RRID: AB_3111412)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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|---|---|---|---|
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| HPAB-1338-FY | Human Anti-MSLN Recombinant Antibody (HPAB-1338-FY) | ELISA, FC | Human IgG |
| HPAB-1339-FY | Human Anti-MSLN Recombinant Antibody (HPAB-1339-FY) | ELISA, FC | Human IgG |
| HPAB-1340-FY | Human Anti-MSLN Recombinant Antibody (HPAB-1340-FY) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-009 | Recombinant Anti-human MSLN VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| HPAB-M0391-YC | Recombinant Llama Anti-MSLN Single Domain Antibody (HPAB-M0391-YC) | ELISA, FC, IF | Llama VHH |
| HPAB-M0392-YC | Recombinant Llama Anti-MSLN Single Domain Antibody (HPAB-M0392-YC) | ELISA, FC, IF | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-201 | Anti-Human Mesothelin Recombinant Antibody (TAB-201) | IP, IF, FuncS, FC, Neut, ELISA, IHC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G052E | Anti-MSLN immunotoxin RG7787 (Fab)-PE | Cytotoxicity assay, Function study | |
| AGTO-G053E | Anti-Msln immunotoxin (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-L035E | anti-MSLN immunotoxin K1 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L063E | anti-MSLN immunotoxin 8B35 (scFv)-PE | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0833CL | Human Anti-MSLN Recombinant Antibody (TAB-0833CL) | ELISA, FuncS, Activ, FC | Human IgG1 |
| TAB-0834CL | Human Anti-MSLN Recombinant Antibody (TAB-0834CL) | ELISA, FuncS, FC, SPR, Inhib | Human IgG1, κ |
| TAB-0835CL | Human Anti-MSLN Recombinant Antibody (TAB-0835CL) | ELISA, FC, FACS, Inhib | Human IgG1, λ |
| TAB-0833CL-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (TAB-0833CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0834CL-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (TAB-0834CL-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0376MZ | Chicken Anti-MSLN Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2302 | Hi-Affi™ Recombinant Rabbit Anti-MSLN Monoclonal Antibody (DS2302AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0646-CN-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0646-CN-S(P)) | WB, ELISA, FC | Human scFv |
| HPAB-0442LY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0442LY-S(P)) | ELISA, FC, IHC, WB | Human scfv |
| HPAB-0443LY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0443LY-S(P)) | ELISA, FC, IHC, WB | Human scfv |
| HPAB-0444LY-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0444LY-S(P)) | ELISA, FC, IHC, WB | Human scfv |
| HPAB-1338-FY-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-1338-FY-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0646-CN-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0646-CN-F(E)) | WB, ELISA, FC | Human Fab |
| HPAB-2735LY-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-2735LY-F(E)) | ELISA, FC | Human Fab |
| HPAB-2736LY-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-2736LY-F(E)) | ELISA, FC | Human Fab |
| HPAB-2737LY-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-2737LY-F(E)) | ELISA, FC | Human Fab |
| HPAB-2738LY-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-2738LY-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-201 | Afuco™ Anti-MSLN ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-201) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0101 | APC-H-2Db/Human Mesothelin (GQKMNAQAI) MHC Tetramer | FCM | |
| MHC-CN0102 | PE-H-2Db/Human Mesothelin (GQKMNAQAI) MHC Tetramer | FCM | |
| MHC-YF238 | A*02:01/Human Mesothelin (SLLFLLFSL) MHC Monomer | MHC Multimer | |
| MHC-YF239 | A*02:01/Human Mesothelin (VLPLTVAEV) MHC Monomer | MHC Multimer | |
| MHC-YF553 | A*01:01/Human Mesothelin (TLDTLTAFY) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY69 | Human Anti-MSLN (clone SS) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC539 | Recombinant Anti-MSLN Vesicular Antibody, EV Displayed (VS-0425-YC539) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4533 | Anti-MSLN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4534 | Anti-Mouse MSLN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4535 | Anti-Rat MSLN Immunohistochemistry Kit | IHC |
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